Liver uptake & ADMET studies

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Liver cell distribution in vivo

The major cell type responsible for liver metabolism of biopharmaceutical compounds are often not hepatocytes, but the lesser known liver sinusoidal endothelial cells (LSECs). Consequently, any liver metabolism studies need to include the LSEC in order to reveal the complete interaction of biopharmaceuticals with liver cells.

With D’Liver’s specialised expertise in isolating highly purified freshly prepared cultures of hepatocytes, Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs) we determine the exact cellular site(s) of liver uptake and metabolism after administration of the compound. We also detect toxicological effects on these cell types. The assays can be performed in fish, mice, rats and pigs.

 

Assays

  • Liver cell type(s) responsible for uptake
  • Distribution among LSECs, KCs and hepatocytes
  • Metabolic fate/degradation
  • Toxicological effects on LSECs, KCs and hepatocytes
  • Labelling of compound(s) with radioisotopes and/or fluorochromes 

Key benefits and features

  • Reveals liver cell types responsible for uptake at an early stage in drug development which enables modifying the compound or terminate the development
  • Encompass all three major liver cell types
  • Results reflect the corresponding events in humans
  • Analyse native compounds or compounds labelled with 13C, 15N, 14C, 3H, 18F, 125I and/or fluorochromes
  • Studies tailored to our customers’ specific requirements
  • Swift response to requests
  • Quality in execution and delivery