In isolated human liver cells
D'Liver has unique expertise in isolating highly purified freshly prepared cultures of hepatocytes, Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs). These cells, isolated by us on a short time scale, have a purity of > 90% and maintain their remarkable physiological scavenger function.
To study intact liver cell uptake functions, freshly isolated liver cells cannot be replaced by any commercial available cell line. No commercial cell lines are able to maintain the highly specific liver uptake mechanisms in cell culture.
Primary LSECs kept in cultures for more than a day, even under optimal conditions, lose their characteristic scavenger function. Replated 'primary LSECs' or cell lines originating from primary LSECs, are useless in studies on LSEC scavenger function.
Drug interaction studies
We determine effectively and reliably the interaction of the compound with freshly isolated human hepatocytes, KCs and LSECs, identifying the compounds that most likely to pass through the liver without being metabolized.
We analyse native compounds or compounds labelled with 3H, 13C, 14C, 15N, 125I and/or fluorochromes.
Our technology enables a time and cost-effective comparison of interaction profiles of several compounds in the same cell system.
Liver tox studies
Drugs may cause harmful effects on liver cells. We perform MTT and Glutathione assays on freshly isolated human hepatocytes, KCs and LSECs for reliable liver tox studies.